RFdiffusion

This workflow uses RFdiffusion for generating protein backbones, which can be done in two main ways: conditioned on a target structure or by scaffolding a known binding motif. It can also be applied to design miniproteins. The sequence is then designed using either ProteinMPNN or its solubility-optimized variant, SolubleMPNN, which can also be used for round-based optimization. The final designs are validated and filtered using AlphaFold2 and other optional metrics, including those derived from molecular dynamics (MD) simulations.

Pipeline

Description

Proteins (90)

EGFR

IL-7Ra