Description
These are the first test sequences for the open-source design method Mosaic. For a detailed description of the protocol we used, see our matching blog post: https://blog.escalante.bio/minibinder-design-is-just-not-that-hard/. In short, we designed minibinders using hallucination with AF2, AF2 + ProteinMPNN (during design), and Boltz2 + ProteinMPNN.
Proteins (20)
id: frozen-panda-maple
IL-7Ra
Strong
3.0e-9 M
True
9.5 kDa
80
id: brisk-goat-snow
IL-7Ra
Strong
1.5e-8 M
True
9.5 kDa
80
id: violet-moth-crystal
IL-7Ra
Strong
1.7e-8 M
True
9.4 kDa
80
id: soft-goat-oak
IL-7Ra
Medium
6.7e-8 M
True
9.9 kDa
80
id: crimson-mole-quartz
PD-L1
Medium
1.0e-7 M
True
9.1 kDa
80
id: jade-ram-plume
PD-L1
Medium
1.3e-7 M
True
9.4 kDa
80
id: noble-moth-lava
PD-L1
Medium
2.7e-7 M
True
9.5 kDa
80
id: young-bear-cypress
IL-7Ra
Medium
3.4e-7 M
True
9.1 kDa
80
id: gentle-raven-marble
IL-7Ra
Medium
4.0e-7 M
True
9.4 kDa
80
id: golden-heron-quartz
PD-L1
Medium
5.2e-7 M
True
9.0 kDa
80
id: crimson-tiger-flint
PD-L1
Weak
1.2e-6 M
True
9.2 kDa
80
id: bright-swan-lotus
IL-7Ra
Weak
2.0e-6 M
True
9.7 kDa
80
id: ivory-mole-wave
PD-L1
Weak
2.9e-6 M
True
9.2 kDa
80
id: steady-bat-crystal
PD-L1
Weak
4.8e-6 M
True
9.3 kDa
80
id: dark-falcon-maple
IL-7Ra
None
--
True
9.4 kDa
80
id: strong-cobra-iron
IL-7Ra
None
--
True
9.4 kDa
80
id: silent-crane-frost
PD-L1
None
--
True
9.3 kDa
80
id: amber-gecko-dust
PD-L1
None
--
True
9.5 kDa
80
id: silent-ibis-onyx
IL-7Ra
None
--
True
9.1 kDa
80
id: small-quail-opal
PD-L1
Weak
--
True
9.2 kDa
80