A broadly neutralizing antibody was optimized into a nanobody using RFDiffusion before ESMfold-suggested mutations were incorporated into designs. Favorable candidates were further modified through rational design where the binding region with the glycoprotein was optimized. Rationally designed candidates were validated using ESMfold, AlphaFold 2 and AlphaFold 3 before submission, ensuring that all candidates had average pTM, pLDDT, and iPTM >80 while possessing favorable PAE.