Generation of 2000 candidates via Boltzgen (length 120-160) Selection of first 30 best candidate binders ranking accordingly to the largest Buried Solvent Accessibility upon binding (DASA) (here top 10) Performing mutagenesis using BeAtMuSiC. Here we use the symmetric version Selecting the mutations that increase the binding affinity more than -0.6 Insert all mutations independently on possible epistatic effects