2VSM (Nipah virus attachment glycoprotein in complex with human cell surface receptor ephrinB2) was relaxed with Rosetta FastRelax (continued to work with the best five outputs -> lowest scores). Missing residues of chain A were added trough aligment to 8JA5 (after FastRelax of 8JA5). After adding the missing residues, the FastRelax was repeated. For each residue the interface dG was calculated with Pyrosetta to choose five Hotspot residues. Bindcraft was used to generate Binders with a length of 25 to 80 aa. Relaxed outputs were sorted by a reweighted score consisting of pLDDT, ipTM, dG and number of relaxed clashes.