Rational Antibody-Based Binder Design (RABBD); Approach: Structure-guided rational design of antibody-like fragments (scFv) and nanobodies (VHH). Analysis of PDB 2VSM (NiV-G/Ephrin-B2 complex). CDR optimization, complementary shape and electrostatics Engineered Scaffold Binder Design (ESBD)'; Approach: Rational design of non-antibody protein scaffolds with engineered binding loops. Structural analysis of binding interfaces and allosteric sites. Loop engineering, avidity optimization, conformational flexibility