BoltzGen’s nanobody-design protocol to target the deep receptor-binding pocket of NiV-G while enabling nanobody refolding during generation, ensuring foldability-preserving constraints and pocket-focused filtering throughout the design process. Binding sites targeted (whole NiV-G-Ephrin-B2 interface): 122..124.140,143..146,185,206,208,209,255,193,295,298,305..308,345,362,394..396,405..411,434..437,461..464,483,485,490,492