Submission 1

Description

We combined the newly released BoltzGen nanobody-design framework with structure-guided filtering and manual interface-safe mutagenesis to generate ten Nipah virus-targeting nanobodies: nine de novo binders aimed at the receptor pocket of NiV-G and one rationally mutated n425 control.

Proteins (10)

TableGrid

XBXevi Biarnés

id: amber-quail-thorn

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.73

87.74

12.7 kDa

116

id: vast-cat-leaf

Binder

Nanobody

BoltzGen

No preview available

Target

Nipah Virus Glycoprotein G

0.71

83.40

13.2 kDa

121

id: jade-falcon-pine

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.67

87.85

12.4 kDa

116

id: ivory-panda-fern

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.45

79.71

12.9 kDa

121

id: hollow-mole-wave

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.32

80.49

12.9 kDa

121

id: lunar-kiwi-marble

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.31

86.21

12.4 kDa

116

id: silver-quail-sand

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.26

84.12

12.7 kDa

118

id: steady-falcon-orchid

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.23

82.49

12.8 kDa

120

id: rough-panda-jade

Binder

Nanobody

BoltzGen

Target

Nipah Virus Glycoprotein G

0.08

85.72

13.5 kDa

123

id: dark-toad-oak

Binder

Nanobody

Structure Guided Mutagenesis

Target

Nipah Virus Glycoprotein G

0.01

88.42

12.8 kDa

119