The BoltzGen binder designs were generated through Tamarind.bio due to low accessibility to GPUs at my institute, using the binding site residues 314,316,317,318,320,341,342,343,367,369,392,393,394,395 with a length range of 70-100 amino acids and 150 total designs across 1 batch. As the flexibility of the BoltzGen platform is quite rigid in fine-tuning parameters, the core rationale about the design comes mostly from literature review of residues involved in Nipah binding with partners. The 5 top-ranked binder were selected based on optimized metrics including design_to_target_iptm, min_design_to_target_pae, design_ptm, representing the best-performing design out of 71 generated candidates (+ visual inspection of predicted complexes).