In vitro SPR validation of SynthIDBio-sequence watermarked binders against VEGF-A, SARS-CoV-2 RBD and PD-L1, from "Function-preserving watermarking of AI-generated proteins" (Nature, https://www.nature.com/articles/s41586-026-10965-y). Source data: https://github.com/google-deepmind/synthidbio (CC BY 4.0). All designs resequence AlphaProteo backbones with ProteinMPNN. Groups: no watermark (T=0.1); SynthIDBio distortionary (T=0.1); SynthIDBio non-distortionary (T=0.5); AlphaProteo parent sequences.
id: swift-kiwi-birch

PD-L1
Strong
7.7e-11 M
True
13.6 kDa
120
id: frozen-jaguar-moss

Human VEGF110
Strong
9.1e-11 M
True
13.6 kDa
128
id: shy-goat-plume

Human VEGF110
Strong
1.5e-10 M
True
12.9 kDa
127
id: pale-panda-maple

PD-L1
Strong
1.9e-10 M
True
13.6 kDa
120
id: amber-lynx-quartz

Human VEGF110
Strong
2.0e-10 M
True
10.5 kDa
94
id: young-shark-opal

Human VEGF110
Strong
2.3e-10 M
True
13.5 kDa
125
id: frozen-falcon-wave

PD-L1
Strong
2.5e-10 M
True
13.5 kDa
120
id: azure-tiger-moss

PD-L1
Strong
2.8e-10 M
True
13.7 kDa
120
id: calm-bison-clay

Human VEGF110
Strong
2.9e-10 M
True
10.6 kDa
94
id: hollow-bear-willow

PD-L1
Strong
3.6e-10 M
True
13.3 kDa
113
id: azure-falcon-ivy

PD-L1
Strong
4.0e-10 M
True
13.3 kDa
120
id: azure-moth-vine

Human VEGF110
Strong
4.5e-10 M
True
10.6 kDa
94
id: pale-dove-moss

Human VEGF110
Strong
5.4e-10 M
True
14.2 kDa
133
id: gentle-moth-leaf

Human VEGF110
Strong
5.7e-10 M
True
10.4 kDa
94
id: strong-cobra-opal

Human VEGF110
Strong
5.7e-10 M
True
10.5 kDa
94
id: scarlet-raven-pine

Human VEGF110
Strong
6.0e-10 M
True
13.6 kDa
125
id: silver-kiwi-quartz

Human VEGF110
Strong
7.3e-10 M
True
13.6 kDa
125
id: pale-ox-lava

Human VEGF110
Strong
7.8e-10 M
True
13.5 kDa
125
id: quiet-hawk-granite

PD-L1
Strong
1.0e-9 M
True
13.6 kDa
120
id: quiet-fox-iron

PD-L1
Strong
1.1e-9 M
True
13.4 kDa
120
id: lunar-cat-cedar

PD-L1
Strong
1.1e-9 M
True
13.8 kDa
120