Miniprotein vast-otter-leaf

Google DeepMind / vast-otter-leaf

Type

Miniprotein

Binding

PD-L1

Medium

Expressed

Yes

Ref

PDW_1554

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (81 AA)

NEAEEDLLDELSVLALQAIQDRDEDKAIEILERMYDIARRSSNEKLKRRAAGLLEALAAIAERKGLTRVAQRARELAAELR

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

5.05pH

Molecular Weight

9.2kDa

Download CIF

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Binding

Yes

Binding Strength

Medium

K_D

3.6×10⁻⁷M

k_on

6.8×10⁴M^-1s^-1

k_off

2.0×10⁻²s^-1

Download raw data

Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (non-distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, non-distortionary keys

temperature0.5

sequenceDesignProteinMPNN