Miniprotein steady-shark-fern

Google DeepMind / steady-shark-fern

Type

Miniprotein

Binding

SARS-CoV-2 RBD

Medium

Expressed

Yes

Ref

PDW_1281

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (123 AA)

KAIEEAGKAAERIEDPDVRGAASLALGAIHAQVRHGGSGGVAAAVAVAAVADGISDNLTDEELRTVATFIVRALERLGLELPSAEELSHELQWVRRAAKDYMTAKELEKFDRISRALLDEVAA

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.95pH

Molecular Weight

13.1kDa

Download CIF

Loading structure viewer...

Binding

Yes

Binding Strength

Medium

K_D

1.2×10⁻⁷M

k_on

2.3×10⁴M^-1s^-1

k_off

2.6×10⁻³s^-1

Download raw data

Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

Loading kinetic curves...

Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (non-distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, non-distortionary keys

temperature0.5

sequenceDesignProteinMPNN