Miniprotein shy-toad-leaf

Google DeepMind / shy-toad-leaf

Type

Miniprotein

Binding

Human VEGF110

Strong

Expressed

Yes

Ref

PDW_1027

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (136 AA)

SKEKEKDRFLELAAQALAALAPHGVDVDRVLAELATIADQLDADELSHLVKNAERLRQALEDAGFSAQQADAVVGAALVSLSGDEKDVELAKEGLAAALGVKASKEEVDRIVSLAEAVLLGAATPEDVVRAVRELL

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.52pH

Molecular Weight

14.3kDa

Download CIF

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Binding

Yes

Binding Strength

Strong

K_D

7.5×10⁻⁹M

k_on

1.3×10⁶M^-1s^-1

k_off

9.4×10⁻³s^-1

Download raw data

Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (non-distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, non-distortionary keys

temperature0.5

sequenceDesignProteinMPNN