Miniprotein radiant-shark-sand | Proteinbase

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Design Methods
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Created by

Miniprotein radiant-shark-sand | Proteinbase

GD

Google DeepMind / radiant-shark-sand

Type

Miniprotein

Binding

SARS-CoV-2 RBD

Strong

Expressed

Yes

Ref

PDW_1144

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (65 AA)

SAVEKAIANAKKGLENAKKDGASELSIRGLESAINLLEEYKAGVVPESLKPDAEDLIKYFSAIKD

Sequence (65 AA)

SAVEKAIANAKKGLENAKKDGASELSIRGLESAINLLEEYKAGVVPESLKPDAEDLIKYFSAIKD

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

5.10pH

Molecular Weight

6.9kDa

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Binding

Yes

Binding Strength

Strong

K_D

3.7×10⁻⁸M

k_on

4.7×10⁵M^-1s^-1

k_off

1.6×10⁻²s^-1

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN (no watermark)

Parameters

backboneAlphaProteo

watermarknone

temperature0.1

sequenceDesignProteinMPNN

Proteins
radiant-shark-sand

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Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM