Miniprotein frozen-wolf-ruby

Google DeepMind / frozen-wolf-ruby

Type

Miniprotein

Binding

Human VEGF110

Strong

Expressed

Yes

Ref

PDW_1011

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (132 AA)

SFKDLSPEEVIDDFVNGLYKLAETLPEAYREELKKLATEIRRAAKARDFERLVELSIKLLQLLVEAEAPPASIAATVAAIVAVLAELKAPLAALRRLKEAVEALAAAVESPRQRGALEAALTIIDQTIEALR

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

5.03pH

Molecular Weight

14.4kDa

Download CIF

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Binding

Yes

Binding Strength

Strong

K_D

1.2×10⁻⁸M

k_on

5.2×10⁵M^-1s^-1

k_off

6.2×10⁻³s^-1

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Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (non-distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, non-distortionary keys

temperature0.5

sequenceDesignProteinMPNN