Miniprotein brisk-deer-marble | Proteinbase

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Design Methods
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Created by

Miniprotein brisk-deer-marble | Proteinbase

GD

Google DeepMind / brisk-deer-marble

Type

Miniprotein

Binding

SARS-CoV-2 RBD

Medium

Expressed

Yes

Ref

PDW_1143

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (65 AA)

SKVEKAIENAKKGLENAIKDGASEESIRGLKSAINLLEEYKNGVVPESLKPDAEDLVDYFSKIKD

Sequence (65 AA)

SKVEKAIENAKKGLENAIKDGASEESIRGLKSAINLLEEYKNGVVPESLKPDAEDLVDYFSKIKD

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.95pH

Molecular Weight

7.1kDa

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Binding

Yes

Binding Strength

Medium

K_D

6.7×10⁻⁸M

k_on

3.7×10⁵M^-1s^-1

k_off

2.3×10⁻²s^-1

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN (no watermark)

Parameters

backboneAlphaProteo

watermarknone

temperature0.1

sequenceDesignProteinMPNN

Proteins
brisk-deer-marble

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Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM