Miniprotein steady-otter-lava

Google DeepMind / steady-otter-lava

Type

Miniprotein

Binding

Human VEGF110

Strong

Expressed

Yes

Ref

PDW_0923

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (132 AA)

SFKEASPEEVVDDFVTGLYKLAEKLPEAAGKKLRELADQLKKAALAKDFEEFVELGVETVELLVEADAASNSIGATVAALVAVLSALQAPLDALQRLLEDIEALAKKVKSENQRAALEAALTIVRETIEALK

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.61pH

Molecular Weight

14.1kDa

Download CIF

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Binding

Yes

Binding Strength

Strong

K_D

2.8×10⁻⁸M

k_on

2.0×10⁶M^-1s^-1

k_off

5.5×10⁻²s^-1

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Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, distortionary keys

temperature0.1

sequenceDesignProteinMPNN