Miniprotein misty-ant-opal

rooshanie ejaz / misty-ant-opal

Type

Miniprotein

Ref

RBX1_l67_s655068_mpnn7

Collections

R[GEM x Adaptyv: RBX1 Binder Design Competition] Submission 1

AGEM x Adaptyv RBX1 Binder Competition: Main Submissions

Sequence (67 AA)

SLEPLVERFNGYLEKGRINDLIRSPDWWKYEDAAVEALMRENGGMPRWEAEHLLHRQMHDAIQNPNA

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

5.21pH

Molecular Weight

7915.79Da

Download CIF

Loading structure viewer...

No experimental data

This protein hasn't been validated in the lab yet.

Design Method

This protein was designed using EBindCraft

BindCraft (Pacesa et al., 2025) with default 4stage_multimer settings. Target: RBX1 RING domain (res 40-108) from PDB 4F52 chain B. Hotspots: A43-44,A46,A55,A86-87,A91,A95-96,A98 (E2/GLMN-binding surface). Binder lengths: 60-140 AA. Sequence redesign: SolubleMPNN. Filtering: default BindCraft filters (pLDDT, i_pTM, i_pAE, Rosetta dG, shape complementarity, clash count, binder RMSD). Executed on NVIDIA A100 80GB GPUs.