Miniprotein brisk-orca-cloud

rooshanie ejaz / brisk-orca-cloud

Type

Miniprotein

Ref

RBX1_l72_s701802_mpnn2

Collections

R[GEM x Adaptyv: RBX1 Binder Design Competition] Submission 1

AGEM x Adaptyv RBX1 Binder Competition: Main Submissions

Sequence (72 AA)

SHHLPPDSPAAREYERVFAETLEEVTGGKERPTREQGIEIINRLMRYFWEHNPEHFELLAWDIWSRVQALIQ

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

5.25pH

Molecular Weight

8627.55Da

Download CIF

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No experimental data

This protein hasn't been validated in the lab yet.

Design Method

This protein was designed using EBindCraft

BindCraft (Pacesa et al., 2025) with default 4stage_multimer settings. Target: RBX1 RING domain (res 40-108) from PDB 4F52 chain B. Hotspots: A43-44,A46,A55,A86-87,A91,A95-96,A98 (E2/GLMN-binding surface). Binder lengths: 60-140 AA. Sequence redesign: SolubleMPNN. Filtering: default BindCraft filters (pLDDT, i_pTM, i_pAE, Rosetta dG, shape complementarity, clash count, binder RMSD). Executed on NVIDIA A100 80GB GPUs.