Miniprotein amber-toad-snow

Google DeepMind / amber-toad-snow

Type

Miniprotein

Binding

Human VEGF110

Strong

Expressed

Yes

Ref

PDW_0939

Collections

DSynthIDBio: watermarked protein binders (Google DeepMind)

Sequence (136 AA)

AREEEKARFLELASESLAALAPHGVNQEAILGELAKIAEQLSADELAQLVDNAEALRAALVDAGFSEDQALAVVGAALASLSGNAEDVRLAKEGLRKILGTEASDEETALVVTEALAVLLGARSAADVVRAIRSLV

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.42pH

Molecular Weight

14.0kDa

Download CIF

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Binding

Yes

Binding Strength

Strong, Medium

K_D

4.9×10⁻⁸M

k_on

1.5×10⁶M^-1s^-1

k_off

7.6×10⁻²s^-1

Download raw data

Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using GDAlphaProteo backbone + ProteinMPNN with SynthIDBio (distortionary)

Parameters

backboneAlphaProteo

watermarkSynthIDBio-sequence, distortionary keys

temperature0.1

sequenceDesignProteinMPNN