The predictions I made began by using the Boltzgen model, which served as the basis for estimating the properties of five mouse Fv antibody candidates. This method involved carefully setting the model parameters to ensure accurate and reliable results specific to these antibodies. The PDB file from the selected samples was inspected manually using ChimeraX, version 1.10. The CDRs were mapped with IgBlast, and site-directed mutations were designed using Python script and evaluating the potential involvement of specific CDRs. New PDB files were generated using Boltz2, and a Python script was used to analyse contacts and predict scores, indicating the best models for interaction with the Rbx1 protein.