De novo RBX1 binders were generated with BindCraft using AlphaFold2-based hallucination and ProteinMPNN sequence redesign. The design strategy focused on the structured zinc-coordinated RING-domain region of RBX1 and deprioritized the disordered N-terminus. Candidate binders were filtered and ranked based on structural confidence, interface plausibility, redesign consistency, and developability-oriented selection criteria.

De novo RBX1 binders were generated with BindCraft using AlphaFold2-based hallucination and ProteinMPNN sequence redesign. The design strategy focused on the structured zinc-coordinated RING-domain region of RBX1 and deprioritized the disordered N-terminus. Candidate binders were filtered and ranked based on structural confidence, interface plausibility, redesign consistency, and developability-oriented selection criteria.