RBX1-01

• Submission order: 1
• Score rank: 1
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: BindCraft de novo mini-protein design
• Description: This mini-protein anchors the submission because it captures the clearest shared signal in the portfolio: a compact scaffold, interpretable main-face engagement, broad surface reach, and enough cross-state consistency to function as a consensus lead rather than a niche experiment. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and follow-up refolds recovered compatible placements in the 3RTR transfer-related conformation, the 8Q7R functional-state conformation, and the 2LGV display-state conformation. What stands out most in the numbers are 13 binder-side residues participating in the modeled interface, a patch that reaches 7 RBX1 residues, broad RBX1 surface coverage of 0.714, high interface confidence at 0.902, strong developability at 0.970, and a composite ranking score of 7.8406. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the evidence is still computational and needs experimental confirmation; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio