spCas9 Target

spCas9 wraps around a guide RNA, forming a ribonucleoprotein that scans DNA for matching sequences. It also checks for a short NGG protospacer adjacent motif, which tells the protein it has found a valid target. Once bound, spCas9 opens the DNA duplex and positions two catalytic centers to slice both strands. Conformational checkpoints and engineered mutations help scientists control off-target activity while preserving efficiency. Allosteric binders, anti-CRISPR proteins, and nanobody partners now give teams manual controls over spCas9 activity. These binders can chaperone delivery, gate editing to certain tissues, or pause the nuclease to reduce off-target breaks during therapeutic development.

spCas9

Description

Proteins targeting spCas9 (20)

Proteins targeting spCas9 (…)

Proteins targeting spCas9 (20)