RBX1-10

• Submission order: 10
• Score rank: 10
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: Proteina seed plus ProteinMPNN redesign
• Description: This Proteina-derived mini-protein was retained to keep scaffold diversity near the front of the submission, rather than letting the main-face route be represented only by BindCraft-style helices. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and current structural support remains centered on that state. The key numerical signals are 6 binder-side residues contributing to the modeled interface, a contact patch that reaches 9 RBX1 residues, moderate RBX1 surface coverage of 0.308, an early but non-zero interface-confidence signal of 0.543, acceptable developability at 0.864, and a composite ranking score of 5.8103. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that current structural evidence is still centered on one RBX1 conformation rather than a recovered cross-state contact pattern; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio