Miniprotein wild-kiwi-opal

Brandon Cantrell / wild-kiwi-opal

Type

Miniprotein

Binding

RBX1

None

Expressed

Yes

Ref

rbx1_gen_0032|helix_v3_E7W,R19Y,R20F,E21I|iPTM=0.949|pLDDT=0.779|composite=0.7390

Collections

B[GEM x Adaptyv: RBX1 Binder Design Competition] Submission 1

AGEM x Adaptyv RBX1 Binder Competition: Main Submissions

AGEM x Adaptyv: RBX1 Binder Design Competition - Results

Sequence (21 AA)

HERTEAWLHMQMELAEEQYFI

Computational Metrics

Experimental Metrics

Design Class

Miniprotein

Isoelectric Point

4.62pH

Molecular Weight

2691.99Da

Download CIF

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Binding

Binding Strength

None

Download raw data

Kinetic Curves (SPR)

Concentration

10.0 nM

31.6 nM

100 nM

316 nM

1000 nM

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Design Method

This protein was designed using BCSAE-Steered ESM2 + Topological Pre-Filtering + Boltz-2

Base model: facebook/esm2_t33_650M_UR50D (650M params, MPS-accelerated) SAE: InterPLM esm2-650m layer 18 (HuggingFace: Elana/InterPLM-esm2-650m) Steering feature: f5922 (helix), clamp=10.0 Topo filter: PH-entropy <= 1.1841 (linear), computed via ripser on Vietoris-Rips filtration Variants per parent: 5 (4 mutations each, temperature=1.0) Boltz-2 for structure prediction Target: RBX-1 RING domain residues 40-108, PDB 1LDK