RBX1-12

• Submission order: 12
• Score rank: 12
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: BindCraft backbone plus ProteinMPNN redesign
• Description: This mini-protein was retained as the cleaner-folding backup for its route, so that lane does not depend on a single geometry. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and current structural support remains centered on that state. The clearest quantitative positives here are a modeled contact patch built around 2 residues on the binder side, a patch that currently touches 1 residue on RBX1, localized RBX1 surface coverage of 0.111, high interface confidence at 0.863, strong developability at 0.950, and a composite ranking score of 5.6479. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that current structural evidence is still centered on one RBX1 conformation rather than a recovered cross-state contact pattern; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio