Miniprotein steady-crow-cloud

Type

Miniprotein

Binding

TNF-α

Strong

Expressed

Yes

Ref

TNFA_OPT_06

Collections

Sequence (138 AA)

YAADTAIALEAVERVEEIALEAIARAKERILSEEEAEEFVERLREVLLETIEALKNLSEEEKAFMLERAERVANNSLYELRWAIEQGSRELNVQAVATAGIRALDVVEEAAMFATDTAGYAAVAAAARAAKAELAAAA

Design Class

Miniprotein

Isoelectric Point

4.45pH

Molecular Weight

15.0kDa

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Binding

Yes

Binding Strength

Strong

K_D

5.1×10⁻¹⁰M

k_on (1:1)

6.9×10⁴M^-1s^-1

k_off (1:1)

4.0×10⁻⁵s^-1

Kinetic Curves (BLI)

Fit Model

Concentration

0.0 nM

10.0 nM

31.6 nM

100 nM

316 nM

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