RBX1-08

• Submission order: 8
• Score rank: 8
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: BindCraft backbone plus ProteinMPNN redesign
• Description: This mini-protein was retained because it carries a narrower interface idea into additional RBX1 states, which makes it more informative than a single-state backup. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and follow-up refolds recovered compatible placements in the 3RTR transfer-related conformation, the 4P5O supplemental conformation, and the 8PQL functional-state conformation. The clearest quantitative positives here are a modeled contact patch built around 2 residues on the binder side, a patch that currently touches 2 residues on RBX1, localized RBX1 surface coverage of 0.222, high interface confidence at 0.882, strong developability at 0.955, and a composite ranking score of 5.9054. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the modeled interface remains relatively narrow, even though the fold-level signal is encouraging; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio