RBX1-05

• Submission order: 5
• Score rank: 3
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: BindCraft backbone plus ProteinMPNN redesign
• Description: This mini-protein stays in the portfolio because it tests a denser and narrower packing mode than the earlier entries, rather than simply repeating the same main-face geometry. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and current structural support remains centered on that state. The strongest parts of the metric profile are 21 binder-side residues participating in the modeled interface, a contact patch that reaches 32 RBX1 residues, localized RBX1 surface coverage of 0.192, high interface confidence at 0.865, strong developability at 0.972, and a composite ranking score of 7.1856. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that current structural evidence is still centered on one RBX1 conformation rather than a recovered cross-state contact pattern; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio