RBX1-07

• Submission order: 7
• Score rank: 6
• Format: Mini-protein
• Primary state: 4F52 main-face state
• Design method: BindCraft backbone plus ProteinMPNN redesign
• Description: This mini-protein was retained because it carries a narrower interface idea into additional RBX1 states, which makes it more informative than a single-state backup. The working model targets the main RBX1 surface represented by the 4F52 Glomulin-blocked reference in the 4F52 main-face state, and follow-up refolds recovered compatible placements in the 3RTR transfer-related conformation, the 8Q7R functional-state conformation, and the 8PQL functional-state conformation. The key numerical signals are 6 binder-side residues contributing to the modeled interface, a patch that currently touches 1 residue on RBX1, moderate RBX1 surface coverage of 0.333, high interface confidence at 0.915, strong developability at 0.963, and a composite ranking score of 6.3102. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the modeled interface remains relatively narrow, even though the fold-level signal is encouraging; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio