RBX1-21

• Submission order: 21
• Score rank: 21
• Format: Peptide probe
• Primary state: 2LGV display state
• Design method: EvoBind peptide design
• Description: This peptide probe was retained because it tests the 2LGV route with a genuinely short-format geometry, instead of asking the portfolio to learn everything from mini-protein scaffolds. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, and follow-up refolds recovered compatible placements in the 3RTR transfer-related conformation, the 4F52 main-face conformation, the 8PQL functional-state conformation, and the 8Q7R functional-state conformation. The key numerical signals are 7 binder-side residues contributing to the modeled interface, a patch that reaches 7 RBX1 residues, localized RBX1 surface coverage of 0.192, an early but non-zero interface-confidence signal of 0.521, and a composite ranking score of 4.7792. Novelty review does not show a disqualifying signal in the present screen set; no strong competing interaction is flagged in the off-target screen; the main caution is that the evidence is still computational and needs experimental confirmation; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio