RBX1-25

• Submission order: 25
• Score rank: 25
• Format: Mini-protein
• Primary state: 2LGV display state
• Design method: Proteina seed plus ProteinMPNN redesign
• Description: This Proteina-derived reserve was kept because it expands the 2LGV lane with compact alternative scaffolds that are sequence-distinct from the earlier long display-state constructs, even though interface evidence is still catching up. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, but the current structural readout is still confined to that primary state and remains preliminary. For now, the clearest measurable positives are tractability and fold quality, with developability at 0.860 and a composite ranking score of 3.3780; direct RBX1-contact evidence has not separated strongly enough to headline this entry yet. Novelty review does not show a disqualifying signal in the present screen set; no strong competing interaction is flagged in the off-target screen; the main caution is that RBX1-facing interface evidence is still too early to treat this as a lead-ready binder, so this entry should be read as an exploratory reserve; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio