RBX1-04

• Submission order: 4
• Score rank: 7
• Format: Peptide probe
• Primary state: 2LGV display state
• Design method: EvoBind peptide design
• Description: This peptide probe was retained because it tests the 2LGV route with a genuinely short-format geometry, instead of asking the portfolio to learn everything from mini-protein scaffolds. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, and follow-up refolds recovered compatible placements in the 8PQL functional-state conformation, the 8Q7R functional-state conformation, the 3RTR transfer-related conformation, and the 4F52 main-face conformation. What stands out most in the numbers are 8 binder-side residues contributing to the modeled interface, a contact patch that reaches 8 RBX1 residues, broad RBX1 surface coverage of 0.833, an early but non-zero interface-confidence signal of 0.505, and a composite ranking score of 6.1494. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the evidence is still computational and needs experimental confirmation; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio