RBX1-22

• Submission order: 22
• Score rank: 22
• Format: Peptide probe
• Primary state: 8Q7R functional state
• Design method: EvoBind peptide design
• Description: This peptide entry remains in the set because it gives the functional-state lane a small-molecule-like probing format that is meaningfully different from the helical mini-protein solutions. The working model targets a functional RBX1 conformation related to the E2-facing interface in the 8Q7R functional state, and follow-up refolds recovered compatible placements in the 4P5O supplemental conformation, the 4F52 main-face conformation, and the 8PQL functional-state conformation. The key numerical signals are a modeled contact patch built around 3 residues on the binder side, a patch that currently touches 2 residues on RBX1, moderate RBX1 surface coverage of 0.333, an early but non-zero interface-confidence signal of 0.433, and a composite ranking score of 4.1449. Novelty review does not show a disqualifying signal in the present screen set; no strong competing interaction is flagged in the off-target screen; the main caution is that the modeled interface remains relatively narrow, even though the fold-level signal is encouraging; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio