RBX1-19

• Submission order: 19
• Score rank: 19
• Format: Mini-protein
• Primary state: 2LGV display state
• Design method: Proteina seed plus ProteinMPNN redesign
• Description: This Proteina-derived display-state entry was retained because it explores a longer 2LGV topology than the peptide probes while still showing enough modeled interface formation to justify a dedicated slot. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, and follow-up refolds recovered compatible placements in the 8Q7R functional-state conformation and the 4P5O supplemental conformation. The key numerical signals are 6 binder-side residues contributing to the modeled interface, a contact patch that reaches 8 RBX1 residues, localized RBX1 surface coverage of 0.192, an early but non-zero interface-confidence signal of 0.475, acceptable developability at 0.880, and a composite ranking score of 5.7267. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the evidence is still computational and needs experimental confirmation; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio