RBX1-28

• Submission order: 28
• Score rank: 28
• Format: Mini-protein
• Primary state: 2LGV display state
• Design method: Proteina seed plus ProteinMPNN redesign
• Description: This long Proteina-derived display-state entry was selected because it asks a different question from the peptide lane: whether a bulkier 2LGV-facing scaffold can make a deeper and more distributed display-state contact pattern. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, and current structural support remains centered on that state. The strongest parts of the metric profile are 12 binder-side residues participating in the modeled interface, a contact patch that reaches 9 RBX1 residues, localized RBX1 surface coverage of 0.154, an early but non-zero interface-confidence signal of 0.391, acceptable developability at 0.860, and a composite ranking score of 3.3780. Novelty review does not show a disqualifying signal in the present screen set; no strong competing interaction is flagged in the off-target screen; the main caution is that current structural evidence is still centered on one RBX1 conformation rather than a recovered cross-state contact pattern; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio