RBX1-18

• Submission order: 18
• Score rank: 18
• Format: Mini-protein
• Primary state: 2LGV display state
• Design method: Proteina seed plus ProteinMPNN redesign
• Description: This long Proteina-derived display-state entry was selected because it asks a different question from the peptide lane: whether a bulkier 2LGV-facing scaffold can make a deeper and more distributed display-state contact pattern. The working model targets the 2LGV display-state RBX1 surface used for orthogonal geometry exploration in the 2LGV display state, and follow-up refolds recovered compatible placements in the 8PQL functional-state conformation. The strongest parts of the metric profile are 14 binder-side residues participating in the modeled interface, a contact patch that reaches 18 RBX1 residues, localized RBX1 surface coverage of 0.231, solid interface confidence at 0.610, acceptable developability at 0.872, and a composite ranking score of 6.5883. Novelty support is acceptable: the UniRef50 30-aa sliding-window screen is clear; no strong competing interaction is flagged in the off-target screen; the main caution is that the evidence is still computational and needs experimental confirmation; the full project rationale and design workflow are documented at https://github.com/tuna010101/RBX1-Binder-Design-Portfolio