We designed a set of Nipah protein binders using our own customized proteinHunter refinement protocol [DOI: 10.1101/2025.10.10.681530v1] with the Boltz structure model. The designs were evaluated based on the confidence metrics scores from the model, plus the number of Alanine residues, and having more negatively charged residues than positively charged ones, since the Nipah virus Glycoprotein G. Then, we did Boltz2 with 5 diffusion samples and 10 recycling steps on the highlighted blue sequence of the Nipah virus Glycoprotein G in the ProteinBase website, together with the designed sequence, and then selected the best ones according to the ipSAE score.

The project was implemented in collaboration with Joan Giménez, Ricardo Gónzalez, and David Masnou.