Given the importance of tetramirazation of the viral G protein to invade it's host, targeting the area which dominates in the polymerazation is quite promising therapeutic strategy(Zhaoqian Wang et al., 2022). According with Wang, Y. et al, this area is accesible for epitope recognision by nanobody, but in a distal location of the ephrin receptor or any other therapeutic devepoled so far (Wang, Y. et al, 2024). The current strategy includes hot spot recognision using as scaffold the n425 single domain antibody of the aforementioned study. More specificaly, de novo designs were generated through RFDiffusion scaffodling and subsequently inverse folding with ProteinMPNN and validation with AF2 initial guess. As template of this campaign was used the crustal structure (ID:8XPY, transmembrane domain not included). Afterwards, designs with Local Interaction Area scores (LIA, Kim et al., 2024) over 1,610 and iPAE lower than 10 were selected for partial diffusion with RFDiffusion to increase their diversity (Temperature = 50). Finally, the design with the higher Boltz2 ipSAE min were submitted to the competition.