These two sequences are variants of the Nipah-protein-binding nanobody crystallized in PDB 8XPS. They were generated by backbone-variant sampling and sequence search using the FoldX force-field rotamer optimization and free-energy evaluation. We then evaluated them with Boltz2 to measure IPSAE.

these variants are predicted with high confidence at another epitope but also well predicted when forced to template + msa. It will be interesting to see where they actually bind.