Partial difusion used to create a ensemble of the interface between target and natural ligand. RFdifusion was used to create new binders while maintaining the interface, but starting the design from diferent initial conformation. The best designs were optimized using a genetic algorithm, with the log likelihood calculated by ESM2 being used as the objective function. https://www.biorxiv.org/content/10.1101/2025.10.29.685431v1